Mesothelial-Mesenchymal Transition (MMT) Mechanism

Mesothelial-Mesenchymal mutation (MMT) MechanismMesothelial kiosks play a vital role in maintaining the body structure of peritoneal tissue paper layer and peritoneal homeostasis. During long term peritoneal dialysis, prolonged exposure of peritoneum tissue layer to peritoneal dialysis solution causes peritoneal fibrosis. Bioincompatible dialysis fluid causes mesothelial kiosks to in allow uncontrolled mesothelial-to-mesenchymal transition triggered by Trans melodic phraseing addition factor-1, which leads morphological and functional disruption of membrane. This literature review is ab verboten mechanism of mesothelial -mesenchymal transition (MMT), myofibroblasts and its business line hypothesis, clock genes and their cross parity with electric organ fibrosis.1.1 Peritoneum anatomyPeritoneum is defined as gliding membrane, composed of mesothelial cadres maintained by basement membrane and thin layer of junction tissue. It consists of two distinct layers of mesothelium, known as parietal peritoneum and visceral peritoneum. parietal peritoneum course of actions the peritoneal perdition and comes from somatic mesoderm of the embryo. Visceral covers the external surface of most abdominal organs and derived from splanchnic mesoderm of the embryo. Peritoneal cavity is potential space among both layers and is comprised of small count of lubricating fluid, overly known as serosal fluid which prevents friction between closely packed organs and allows freehanded exertion of them. 1,2 The primary function of peritoneal membrane is to abide support to abdominal organs. It alike acts as passage to their blood vessels, lymph vessels and nerves.Peritoneal cavity is rich in number of cells like mesothelial cells, fibroblasts, endothelial cells, adipocytes and resistive cells like macrophages, T cells, B cells, mast cells. Along with cells, they contain cytokines and growth factors, like interleukin-1 (IL-1), Transforming growth factor-1 (TGF-1), Fibrob last growth factor (FGF), and Hepatocyte growth factor (HGF). All through the development butt on of peritoneal fibrosis, peritoneal cells impart experience substitutes and some of the pro inflammatory factors which act as inducers to induce fibrosis and inflammatory serve upes. In this intentional project, we be going to focus on mesothelial cells along with Transforming growth factor- (TGF-1), which is considered as main mediator of organ fibrosis development.1.2 Peritoneal Fibrosis cause by peritoneal dialysisPeritoneal Dialysis, an alternative to haemodialysis is being used ab divulge more(prenominal) than three decades ago to treat end stage renal disease. During this treatment, PD solution is introduced into the abdomen with the coope tempo of tube also known as catheter. This dialysis therapy uses peritoneum membrane as semi-perme fit membrane and principle of diffusion, to diffuse drive out from underlying blood vessel into the solution.PD solution is major drawback of this treatment which contributes to development of peritoneal fibrosis in patients due to its bioincompatible nature. Long-time exposure to hypertonic environment of PD solution with racy glucose concentration, acidic pH, presence of Glucose debasement Products (GDPs) and Advanced Glycation end-products(AGEs) make functional and structural alterations in peritoneum membrane. 43Other factor which triggers fibrosis change in peritoneum membrane during dialysis is peritonitis. Peritonitis is inflammation of peritoneum which is caused by infection that can occur either by using unsterile catheter tube, manipulation which lead to introduction of micro-organism into abdominal cavity. Interesting research was through with(p) by Troidle and team, where they reported that inflammation caused by Gram-positive bacteria in patient peritoneum ar better managed than the one caused by gram-negative. 81Numerous clinical studies carried out to necessitate changes that occur in peritoneu m of patients undergoes peritoneal dialysis. Till date, largest peritoneal biopsies study was carried out by William and colleague on total of 212 subjects, out of which 9 were approach pattern indivithreefolds, 48 undergoing hemodialysis, 25 uremic and one hundred thirty subject undergoing PD. 44 There submesothelial thickness was measured, also were checked for vasculopathy and general morphological features. For normal individuals, submesothelial thickness was 50 m, patients undergoing hemodialysis and uremic patients was 140 m and 150 m respectively and for PD patients, thickness was 270m. analogous thickness of uremic and haemodialysis patients indicates that uremia can cause change in the peritoneal membrane. tax of submesothelial thickness change magnitude to 700 m in long term PD patients, which directs towards fibrotic response of peritoneal to PD. They were also able to identify vascular changes including increasing hyalinization leading to vasculopathy. These finding s were confirmed by Plum et al and were able to identify link between increasing thickness of submesothelial layer and increasing transport rate of solutes. 451.3 Mesothelial Cells Structural and Functional PropertiesEven though being mesoderm origin, mesothelial cells possess various characteristics of epithelial cells. Normally, mesothelial cells are squamous, and flattened in morphology. They are rich in cell organelles, microvilli and cilia are present on the surface. term cilia aim to regulate surfactant secretion, microvilli functions to entrap water and serous exudates in order to guard cell surface from frictional injuries. 5Glycocalyx created by glycosaminoglycan, proteoglycans, and phospholipids surround mesothelial cells and fork over dual function. They form non-sticky slippery surface which supports intracoelomic movement and also provide protection against scratch.Mesothelial cells are specialized cells which plays key role in peritoneal homeostasis and maintains the rightfulness of the peritoneal membrane. The critical roles of mesothelial cells in peritoneum are to secrete and synthesize growth factors, cytokines and chemokines in peritoneal cavity by transport of fluid and solute across the membrane. 6Mesothelial cells are considered as cells that are first in line in bodys defense system and they are able to sense and responds to signals within their microenvironment. They actively participate in deposition and clearance of local fibrin in the serosa membrane cavities. After mechanical injuries and infections, their fibrinolytic activity is of import to prevent and remove any fibrin deposits that may forms. 7 Peritoneal membrane does act as selective permeableness barrier to the channel of plasma proteins due to existence of anionic sites on mesothelial cells which in expiration provide net negative charge to the peritoneum membrane.In normal conditions, mesothelial cells are identified on its ability to posit cell markers like o ccludin, claudin-1, ZO-1, Wilms Tumor-1, and E-cadherin. When mesothelial cells undergo Mesothelial-to-mesenchymal transition which we will be explained in detail later they either unable to express them or their level face mitigates. Therefore, its important to know about all mesothelial cell markers for easy identification within pool of cells.1.3.1 Cell markersCell markers are defined as molecules with classifiable genetic and biochemical characteristic. These cell markers are present in cells and can be either proteins, peptides or carbohydrates and mainly present in the cells. They are used to distinguish between different cell types. some(prenominal) of cell markers are specific to cell types and some are disease specific. For instance, Cancer antigen 125, calretinin and vimentin are tumour markers that are expressed by tumor cells. Some of mesothelial cells markers are positive while some are negative. Markers like hyalurnon, phospholipids, protein phosphate inhibitors-1 are formed by mesothelial cells itself. Table below shows previously studied markers for mesothelial cells and their function. confuse- Cell Markers for mesothelial cellsMARKERFUNCTIONSOURCEREFERENCEAUA1Immunocytochemical marker of epithelial cells homo22CalretininDiagnostic irradiation for Hirschsprung decreed marker of mesothelioma gentleman2372Cancer Antigen(CA) 125Tumor markerSpecific marker for mesothelial cell massHuman2473CD44Cancer stem cell markerHuman25CD44HReceptor for hyaluronic acidPositive mesothelial markerHuman2674CytokeratinsEpithelial specific markerExpressed by mesothelial cellsHuman hobo2775Cytokeratin 5/6Mesothelioma markerHuman28D2-40Potential diagnostic marker of mesotheliomaHuman29DesminPromising differentiating marker of thermolabile mesothelium from malignant epithelialHuman30E-CadherinCell- cell adhesion markerUsed for differentiating clement reactive mesothelial cellsHuman31HBME-1Negative mesothelial marker in pleuraSensitive mesothelial marker in pleu raHumanHuman3233HyalurnonFormed by Mesothelial cellsPositive mesothelial peritoneal markerHuman34KeratinPresent in extreme amount in mesothelial cellsLow amount or negative in mesotheliomaHumanHuman3536Keratin 7 (K7) passing expressed by mesothelial cellsHuman37Mcp130Diagnostic tool for mesothelioma as well used for ovarian carcinomasHumanRat38ME1/ME2Used for identifying both mesothelioma and normal mesothelial cellsHuman22MesothelinExpressed by both reactive mesothelial cells and epithelioid mesotheliomaHuman39N-CadherinHuman mesenchymal stem cell markerAble to differentiate cardiomyocyteHumanHuman3076PhospholipidsFormed by mesothelial cellsAlso known as positive marker for mesothelial cells in peritoneal fluidHumanHuman3424ProteinPhosphate Inhibitor-1 determine as novel positive marker of mesotheliumMouse40ThrombomodulinPositive, specific and sensitive marker for mesothelial cells.Human28Vimentin economic aid in identifying mesothelial origin cellsHuman41Wilms Tumour 1 (Wt1)Marker used for differentiate between benign and epithelioid mesotheliumRegulates transition of mesothelial into myofibroblastsHumanHuman42772.0 Fibroblasts in peritoneumTo amend injuries in organ and tissues, cell response it in form of fibrosis. Resident fibroblast states extracellular matrix and collagen. Myofibroblasts are the cells that are produced for carrying out the process of wound better. In general, myofibroblast are gone through the process of apoptosis subsequently completing healing process. It has been suggested that they become doggedness after its mechanism fails and leading to more deed and contraction of Extracellular matrix (ECM) and collagen causing fibrotic diseases.2.1Myofibroblasts in fibrosisMyofibroblasts are defined as specialized subset of cells, which are considered as the key cells in tissue fibrosis. 9 electron microscopy showed that myofibroblast have disorganized membrane (changes that occur in actin cytoskeleton), showed presence of stress fibers, m icrofilament bundles and also showed adhesions in the fibroblastic cells of granulation tissue. 10 Further electron microscopy showed the presence of gap junctions which connects myofibroblasts, thus strengthening the idea of resemblance between myofibroblasts and cool energy cell. Myofibroblast main functions are wound healing and connective tissue re placeing.2.2 Fibroblast differentiation into MyofibroblastTo study and learn functions of myofibroblast, a series of both in-vivo and in-vitro experiments were carried out by Hinz and Gabbiani. 15 They defined the origin of myofibroblasts as a result of 2 step differentiation of fibroblast process. 78 In initial step, fibroblasts differentiate into proto-myofibroblasts during mechanical stress and results in production of stress fibers which contains cytoplasmic actin. They also function to express and establish cellular fibronectin and are also enable to produce contractile force. Term proto-myofibroblast was given to these activa ted fibroblasts to differentiate them from inactive fibroblasts which are unable to produce contractile force. TGF-1 along with ED-A fibronectin, under mechanical stress boosts further differentiation of proto-myofibroblast into myofibroblast. They are reason by extensive -smooth muscle actin level of expression and produces stronger contractile force than proto-myofibroblasts. This is also contributing into connective tissue remodeling. Gabbiani and Hinz also stated that unlike dermal wound healing, in pathological wound healing myofibroblasts do not vanish through apoptosis instead they lead to tissue deformation. In fact, contractures triggered by them are recognized characteristic of organ fibrosis. 792.3 Hypothesis surrounding Myofibroblasts originThe origin of Myofibroblasts in fibrotic tissue has been much debated, with disparate findings likely to stem from the range of different fibrotic diseases investigated and the array of technical approaches taken. 11 some of the pro posed theories have different cellular sources and mechanisms which lead to their origin (Figure 3).Previous studies on dermal fibrosis proposed that mesenchymal-like cells known as pericytes are nonmigratory source of myofibroblasts. Immunohistochemistry and double immunofluorescence of patients biopsies showed phenotype link between pericytes and myofibroblasts. 80 It has been described that pericytes can differentiate into osteoblast, adipocytes, and smooth muscle cells. Research carried out by Lin et al., on Col1a1-GFP mice and Humphreys et al., using FoxD1-Cre knock-in mice, both transgenic mice with aim to identify pericytes in genetically labelled renal stroma to suggest that major source of myofibroblasts is pericytes, a contractile cell. 12, 13 A limitation of the aforesaid(prenominal) studies is the lack of lineage tracing strategies and the use of non-specific markers to identify pericytes. Markers such as CD73, PDGFR- (Platelet-derived growth factor sensory receptor- ) and neuronic/glial antigen 2 (NG2) used in these studies are also expressed by resident fibroblasts.endothelial to mesenchymal transition (EndoMT), similar process to EMT can be another potential source myofibroblasts. Study done by Zeisberg on three independent mouse models with chronic kidney diseases to study renal fibrosis found that EndoMT may contribute in fibrosis as about 30-50% myofibroblasts were able to express -SMA and fibroblast-specific protein-1(FSP-1) and also endothelial cell markers CD31. 16 This indicates myofibroblasts may originate from endothelial cells. Myofibroblasts may also be derived from bone marrow. An elaborate series of studies carried out by Direkze et al., used molecular staining techniques for the detection of Y chromosome markers in irradiated female mice which were transplanted with male bone marrow. 18 They used skin wounding and paracetamol administration to create selection pressure to encourage engraftment of bone marrow derived myofibrobla sts. They were able to identify myofibroblasts in the gut through their ability to express of alpha- smooth muscle actin and their morphologies. These findings are polemic however, as other groups have failed to identify BM derived myofibroblasts.A recent study carried out by Yi Ting Chen et al., did genetic fate mapping of Wilms Tumor-1 positive cells on peritoneal fibrosis models. Fibrosis was induce either by dialysis solution, overexpression of TGF-1, or sodium hypochlorite. 19 Results of this study postures as a challenge to the significance of mesothelial cells as apparent source of myofibroblasts. It showed rather than mesothelial cells, underlying submesothelial resident fibroblast give rise to collagen producing myofibroblast. Results of genetic fate mapping of WT1+ mesothelial cells showed that after peritoneal injury, remaining mesothelial cells repair the disrupted peritoneal membrane by contributing to remesothelialization, but do not undergo MMT. While previous studi es used Wt-1 as a biomarker and Cre recombinase to trace the fate of mesothelial cells. This makes it patent that Chen finding still needs to be confirmed. This study also The last proposed theory for origin of myofibroblast is epithelial-mesenchymal transition.Epithelial-Mesenchymal transition (EMT) is comm single defined as process during which transition to mesenchymal cells, epithelial cells lose their cell-cell adhesion and their polarity. Followed by obtaining mesenchymal markers like FSP 1, HSP47, -SMA and vimentin but loses epithelial markers. 20 Myofibroblasts derived from epithelial cells through EMT proposed theory got strong piece of evidence from immunohistochemical study of peritoneal fibroblasts of continuous ambulatory peritoneal dialysis (CAPD) patients carried out by J-Heffernan et al. 14 Biopsies samples were taken from CAPD patients and divided into four groups normal (I), non CAPD uremic (II), CAPD uremic (III) and non- renal patients with inguinal hernia (IV). Markers like desmin, E-cadherin, cytokeratins, -SMA and also CD34 were analysed for expression by mesothelial cells and resident fibroblasts. Group I and II did expressed CD34 but no other markers. The reverse pattern was observed in other two groups. Degree of peritoneal fibrosis is connected with loss of CD34 expression. Myofibroblasts appear to originate from both by activations of resident fibroblasts and by mesothelial-to-mesenchymal transition of mesothelial cells.3.0 Mesothelial-Mesenchymal Transition (MMT)Under regular conditions, Mesothelial-to-mesenchymal transition of mesothelial cells is an autoregulated biological process which functions to repair tissues but under uncontrolled situations (Long term Peritoneal dialysis) they can cause Peritoneal fibrosis.3.1 Stages of MMTMesothelial -Mesenchymal Transition (MMT) is similar process to Epithelial Mesenchymal Transition (EMT). Defined as a step-wise and complex process where mesothelial cells undergo modifications in th eir architecture and molecular functions. 21 48 Simple steps in which process of MMT occurs starts with the downregulation of intracellular adhesion molecules causing loss of microvilli, apical-basal polarity and disrupting intracellular tight junction. These transition undergoing cells adopt front to back polarity, have increased in their migration capacity, morphological changes and are able to express -SMA. The main objective of this process is to repair wound tissues and also play role in process like migration of cells, inducing neoangiogenesis and production of extracellular matrix.Molecules and planetary house pathways that play role of either inducers or blocker during the process of MMT are TGF-1 induced polarity pathways and BMP-7 signaling pathway. They are member of superfamily of growth factors, along with activins/inhibin and growth and differentiation factors (GDFs). Main attention is on growth factors because equilibrium between these factors maintains the epithel ial like-phenotypic characteristics of Mesothelial cells and also in acquiring mesenchymal cells characteristics.Studies not only showed that high levels of cyclooxygenase (COX)-2, CTGF and VEGF are expressed by mesothelial cells that undergo MMT. But also that there is connection between expression of these molecules and rate of transport of solute across peritoneal membrane. These molecules are also caught up into fibrotic, angiogenic and also in inflammatory responses. 4950513.2 Cell Signaling PathwaysActivation of TGF- , master molecule in peritoneal fibrosis during Peritoneal Dialysis, starts MMT signalling cascade down with activating receptors that triggers smads- dependent (GSK-3, -catenin, Lef-1/Tcf, integrin-linked kinase) and smads-independent signalling (H-Ras/Raf/ERK) pathway. 48525354Smad are intracellular proteins that functions to transfer signals from TGF- to nucleus for gene transcription. Smad dependent signalling pathways activates when TGF- and BMP-7 binds with their respective receptor type II (TGF R-II and BMP R-II respectively). The binding leads to activation of receptors type I of TGF and BMP-7. These receptors type I functions to carry out phosphorylation process. TGF R-I phosphorylates Smad2 and Smad3 in TGF- pathway and Smad1, Smad5 and Smad8 by BMP R-I. These activated Smads forms heterodimers with Smad4. Followed by translocation of these complexes into the nucleus where they function to activate genes involved in either process of MMT and rMMT (Fig. 5) Presence of few other BMP-7 modulators may affect the signalling of BMP-7. Smad dependent signalling pathway of TGF- and BMP-7 are controlled by Smad7 and Smad6 respectively.In case of Smad independent pathway of TGF- and BMP-7 involves a protein kinase TAK1, which functions to regulate processes like transcription and apoptosis. In TGF- signalling pathway, TRAF6 initiates activation of TAK1 which further leads to activation of p38 and MAPK-JNK signalling pathway. Receptor type I of TGF- phosphorylates Shc, which helps in activating MAP kinase ERK1/2 signalling pathway. p38 pathway act as blocker of mesothelial cells conversion to mesenchymal. Maybe its due to the fact that XIAB, a protein belong to the family of inhibitor of apoptosis protein helps BMP-7 to activate p38 signalling pathway. TGF- also encourages PI3-K/Akt pathway by inactivating GSK-3, to stabilize -catenin and snail and to activate mTOR. This results in jam of -catenin into the nucleus, where they interact with LEF1/TCF, in order to feed into Wnt signalling pathway. They also contribute in the transcription of mesenchymal-related genes. Furthermore, localisation of snail into the nuclear encourages transcriptional repression of E-cadherin and several other adhesion molecules.Smad dependent or independent pathway, both depicts the fact that TGF- plays a key role in developing fibrosis by promoting mesothelial to mesenchymal transition. Another fact is BMP-7 plays role of antagonist to TGF- . uttermost(a) studies has used TGF-1 as their target goal to design alterative treatment for peritoneal fibrosis. Most of them intend to control TGF-1 activity either via medicine or synthetic proteins design specifically for this purpose.3.3 Evidences of Mesothelial-mesenchymal transition (MMT)There are more than enough studies results which clearly supports the process of MMT occurring fibrosis but in 2003, Yanez-Mo et al., print breakthrough in-vitro and in-vivo study where they were able to demonstrated MCs undergoes mesenchymal conversion. 46. Primarily in vitro studies were carried on by isolating mesothelial cells from patients undergoing peritoneal dialysis. These isolated cells were characterized using immunostaining, western blotting, flow cytometry and reverse-transcriptase polymerase chain reaction. Mesothelial cells from omentum from healthy persons were used as control and all results were compared. Morphological analysis sample showed varies morphologies ranging from cobblestone like appearing cells to fibroblast like cells. Mixed morphology and non-epithelioid were also observed in few samples. Level of E-cadherin, cytokeratin and vimentin were analyzed for the mesothelial-to-mesenchymal transition in mesothelial cell cultures. Results demonstrated noticeably low level of E-cadherin and cytokeratin expression in both cobblestones like cells and non-epithelioid cell as compared to omentum cell cultures. In non-epithelioid cells, vimentin has high expression level. Expression of snail and E-cadherin in mesothelial cells isolated from effluent and omentum were analyzed by the process of RT-PCR. While in omentum mesothelial cells, showed no presence of snail mRNA but increase in expression of these messenger RNA in effluent derived mesothelial cells. These results were established after carrying out in vivo studies of peritoneal biopsy samples.With dual aim of studying anatomical changes in peritoneal membrane and correlating them with functi onal parameter, Del Peso and team carried out a clinical study. 47 They used peritoneum biopsies taken from thirty-five steady patients which were still taking peritoneum dialysis treatment form last 2 years. They observe loss of mesothelial layer in about 74% patients, fibrosis in 46%, 17% showed presence vasculopathy. With submesothelial cytokeratin staining, 17% showed evidence of EMT. Interestingly, they found that myofibroblasts were present in all patients with EMT but only in 36% without EMT.3.4 Potential therapeutic approaches to reverse MMT (rMMT)Several studies describe therapeutic strategies which are targeting anti-EMT of Mesothelial cells are emerging as promising treatment against fibrosis development. Tamoxifen is a drug used for treating breast cancer. The estrogen antagonist is oestrogen receptor and their activity affects action of TGF- 1 has been reported. In year 1991, Clark and team showed noticeable decrease in level of peritoneal fibrosis, when given orally t o peritoneal fibrosis patients.Loureiro and team also used this drug to study its nub in early stage of PD induced peritoneal fibrosis. 61 Theyused PD model of mice for in-vivo study and along with that they also did in-vitro study. In vitro studies were carried out with mesothelial cells (MCs) derived from omentum and effluent. Mesothelial cell sample cultures were checked for any change in morphology and also level of E-cadherin expression after treating them with Tamoxifen. For effluent derived MCs, MMT was not reversed by Tamoxifen but some mesenchymal molecules expression was reduced. In mice PD model, they confirmed that MMT and thickness of peritoneal membrane increases parallel. They also observed that Tamoxifen does reduce thickness of peritoneal membrane and improves its function.They also administrated P17 and P144, synthetic peptides that were designed to bind with TGF-1 directly. 62 It has been reported that in organs like liver, heart, skin these peptides have shown s trong antifibrotic effect by blocking biological function of TGF-1.Bone morphogenic protein-7 (BMP-7) and Hepatocyte growth factor (HGF), are two molecules that were successfully demonstrated their ability to inhibit and reverse process of Mesothelial to mesenchymal transition and renal fibrosis in animal models by X Y D et al., and Yang et al. 64 65In vitro study carried by Yang and team, where they used histone acetyltransferase inhibitor (HAT) C646 in EMT model of HPMCs. 66 EMT was induced by treating with high glucose. C646 treatment of EMT model, found TGF-1/Smad3 signalling was blocked. This might provide with a novel therapeutic treatment for peritoneal fibrosis.Even though most of the therapeutic studies focused on mesothelial mesenchymal transition process and TGF-1, there are other factors that contributes to loss of functional properties of peritoneum. Thus promotin